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Extra resources for Chitosan for Biomaterials II
2 Amphiphilic Chitosan Derivatives . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Chitosan-Based Hydrogels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Biomedical Applications of Chitosan and Chitosan Derivatives . . . . . . . . . . . . . . 1 Gene Delivery by Chitosan and Chitosan Derivatives . . . . . . . . . . . . . . . . 2 Chitosan Derivatives for the Delivery of Poorly Soluble Drugs .
The underlying mechanism is that the poly-alcohol group of b-glycerophosphate cuts off the chitosan chain, accelerating the formation of a hydrophilic shell around the chitosan molecule, and thus improving the chitosan chain protective hydration, which prevents the associative effects at low temperatures and neutral pH. However, with an increase in temperature, hydrophilic interactions and hydrogen bonding start playing an important role and trigger physical crosslinking throughout the whole solution, starting the gelation process .
The percentage of deacetylated primary amine groups along the macromolecular chain) 30 R. Riva et al. determines the positive charge density of the polymer and, consequently, influences the electrostatic interactions with nucleic acids. To ensure a good complexation, the deacetylation degree must be higher than 65% . Thibault et al. also reported that complexes with a greater degree of deacetylation showed a higher level of binding to cells, resulting in enhanced uptake . Another physical characteristic to be considered is the molecular weight of chitosan, which influences the size and the stability of the nanoparticles.