By Max Gerson
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Extra resources for Gerson Therapy Handbook: Companion workbook to A Cancer Therapy- Results of Fifty Cases
R. and Satchidanandam, V. (1996) Analysis of a genomic DNA expression library of Mycobacterium tuberculosis using tuberculosis patient sera: evidence for modulation of host immune response. Infect. Immun. 64, 3765–3771. 5. Chen, X. , Lun, Z. , and Fung, M. C. (2001) High-level expression and purification of immunogenic recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli. Protein Expr. Purif. 23, 33–37. 6. Gao, B. and Tsan, M. F. (2003) Endotoxin contamination in recombinant human heat shock protein 70 (Hsp70) preparation is responsible for the induction of tumor necrosis factor alpha release by murine macrophages.
McAdams, H. , Fraser, C. , and Shapiro, L. (2000) Global analysis of the genetic network controlling a bacterial cell cycle. Science 290, 2144– 2148. 8. Schoolnik, G. K. (2002) Microarray analysis of bacterial pathogenicity. Adv. Microb. Physiol. 46, 1–45. 9. Banerjee, N. and Zhang, M. Q. (2002) Functional genomics as applied to mapping transcription regulatory networks. Curr. Opin. Microbiol. 5, 313–317. 10. Conway, T. and Schoolnik, G. K. (2003) Microarray expression profiling: capturing a genome-wide portrait of the transcriptome.
This goal was reached by the combination of isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) for two-dimensional electrophoresis (2-DE) (3,4). With this improvement, several hundred proteins were separated within one 2-DE gel. The method was further optimized for high resolution, and at present more than 10,000 protein species may be separated within large (30 × 40 cm) gels (5). In 1995 the term proteome was defined: the proteome refers to the total protein complement of a genome (6).