By Ravin Narain
Polymers and Nanomaterials for Gene Therapy presents the most recent info on gene remedy, a subject that has attracted major recognition during the last 20 years for the remedy of inherited and purchased genetic ailments. significant examine efforts are at present keen on designing appropriate service vectors that compact and guard oligonucleotides for gene therapy.
The e-book explores the newest advancements within the box of polymer technology and nanotechnology, and the way those developments have helped within the layout of complicated fabrics. Non-viral vector structures, together with cationic lipids, polymers, dendrimers, peptides and nanoparticles, are power routes for compacting DNA for systemic supply. in spite of the fact that, in contrast to viral analogues that experience no trouble in overcoming mobile limitations and immune safety mechanisms, non-viral gene companies constantly express major lowered transfection potency because of various additional- and intracellular hindrances. hence, biocompatibility and power for large-scale creation make those compounds more and more appealing for gene therapy.
This publication comprises chapters at the engineering of polymers and nanomaterials for gene treatment, and the way they could shape complexes with DNA and steer clear of either in vitro and in vivo obstacles. different chapters describe in vitro, ex vivo, in vivo gene remedy stories, and the present concerns affecting non-viral gene therapy.
- Explores present demanding situations within the examine of genetic diseases
- Discusses polymers for gene treatment and their functionality in designing complex fabrics
- Provides examples of natural and inorganic nanomaterials for gene therapy
- Includes labeling, concentrating on, and assays
- Looks at characterization, physico-(bio)chemical homes, and applications
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Additional resources for Polymers and Nanomaterials for Gene Therapy
For example, PEI/DNA complexes enter into COS-7 cells mainly via the clathrin-dependent pathway . In another study, macropinocytosis was found to be a more effective pathway than endocytosis for PEI/DNA complexes in CHO and Hela cells . Caveolae, which is necessary for caveolae-mediated endocytosis, are rich in vessel wall lining of endothelial cells. Therefore, caveolae-mediated endocytosis is important for these endothelial cells . Uptake of these complexes alone is not sufficient for transfection.
3 Shape Few studies have been done to relate shape and uptake pathway selection of non-viral complexes. Uptake of protein-coated spherical gold nanoparticles was found to be higher than rod-shaped ones in Hela cells . Since DNA/non-viral vector complexes are heterogeneous in shape, its role in selection of uptake pathways is difficult to predict. The shapes of these complexes depend on the experimental conditions . 4 Cell type Another important factor to be considered for transfection study is cell type.
59] Kunath K, von Harpe A, Fischer D, Petersen H, Bickel U, Voigt K, et al. Lowmolecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine. J Control Release 2003;89:113–25.  Godbey WT, Wu KK, Mikos AG. Poly(ethylenimine) and its role in gene delivery. J Control Release 1999;60:149–60.  Zhang ZY, Smith BD. High-generation polycationic dendrimers are unusually effective at disrupting anionic vesicles: membrane bending model.