By Edward Gurr
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Additional info for Synthetic Dyes in Biology, Medicine and Chemistry
Sudan blue is a solvent dye of the anthraquinone group. It has a number of uses as a biological stain; for example, Gurr (1958, 1962) describes its use in demonstrating degenerating myelin. This or a very similar dye has been used in studies of sebaceous glands; in the anatomical study of rubber deposits in Guayule, etc. (see Gurr, 1960, 1965). 28 SYNTHETIC DYES The commercial dye is used for colouring petrol (gasoline); for the mass coloration of cellulose acetate and polystyrene; for colouring soaps, etc.
3 5 3 18-2 16I A - - 4 ιl2 •2 10- < 0 8 - 8 \ι 637 / 0 4 2 \ \ . 325 -20 \ ^ oL—I I g H / - I Χ / 0-6- 0 - 10 V -40 60 ν 80 1 I 350 400 I — 1 450 500 Wavelength (nm) 1 550 1 600 1 650 J 1 0 0u 700 u C 1 3H 1 2N 3 S C 1 Molecular weight 278: Anionic weight 243 SOLUBILITIES 0 Water R+ Ethylene glycol R+ Acetone Ρ Glycerol H Pyridine M Ethanol RMethyl acetate PBenzyl alcohol M P Kerosine — Olive oil — Cellosolve R + Xylene — Chloroform (pure blue) Methyl benzoate s Dioxane — ° Distilled or deionized.
These include Windle et al. (1943), Clark and Sperry (1945) and Anderson (1954), who used dye for neurological material; and Klinger and Ludwig (1957), who used it in their universal stain for the Sex Chromatin Body. Gurr (1962) describes a number of staining methods in detail with citations to the original papers. Among these methods are: M. Change (1936), nerve cells and fibre tracts; Mallory (1938), demonstration of malignant cells in biopsy material; Bensley, (1934), demonstration of acid mucopolysaccharides.