By Ruth Hogue Angeletti
This article offers fresh advances in protein chemistry technique, together with protein sequencing research, high-sensitivity protein and peptide separations, artificial peptides, mass spectral research of proteins and peptides, and protein folding. it's comb-bound for ease of use
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Extra info for Techniques in Protein Chemistry III
Since our main interest is peptide sequencing, we tested only the arylamine-PVDF on the 477A sequencer. Covalent attachment was expected to improve sensitivity and shorten cycle times due to efficient removal of side products and complete extraction of amino acids. High repetitive yields can be obtained, since peptide wash-off is eliminated. Analysis of post-translational modification sites is possible due to flexibility of solvent use (Aebersold, 1990). , 1991). Here we describe optimized conditions for use of solid phase support on the 477A sequencer, and we will discuss the benefits of this combination.
Several members of the authors' laboratories contributed to various aspects of this project, including: Mark Frazier (Washington University School of Medicine, Protein Chemistry Laboratory) for synthesizing the peptide; Richard Thoma for amino acid analysis of the final test sample and David McCourt (Washington University School of Medicine, Howard Hughes Medical Institute) for initial sequence analysis; Brady Stoner and Rodney Prell (Medical College of Wisconsin) for preparation of the test sample; Jean Shelton and Peggy Vroman (Texas College of Osteopathic Medicine) for data entry; Carolyn Moomaw (University of Texas Southwest Medical Center, Howard Hughes Medical Institute) for sequence analysis of the test sample; and Lorraine Whiteley and Pat Parvin (Washington University School of Medicine, Howard Hughes Medical Institute) for expert secretarial assistance.
1990) Nature 343, 291-292. , M. Girardi, and J. Mark (1991) in Techniques in Protein Chemistry II (JJ. Villafranca, ed) pp. 75-78, Academic Press, San Diego. , DJ. Pappin, J. Mark, R. Aebersold, and H. Koster. (1991) Analytical Biochemistry 194, 110-120. A. (1971) Eur. J. Biochem. 20, 89-102. Tempst P. and L. Riviere (1989) Anal. Biochem. 183, 290-300. Evaluation of the Blott Cartridge for Enhanced Gas Phase Sequencing at Maximum Sensitivity David F. Reim, Peter Hembach, and David W. Speicher Wistar Institute, Philadelphia, PA I.